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KMID : 0380220070400010082
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 1 p.82 ~ p.87
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Enhanced Expression of High-affinity Iron Transporters via H-ferritin Production in Yeast
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Kim Kyung-Suk
Chang Yu-Jung
Park Chung-Ung
Seo Hyang-Yim
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Abstract
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Our heterologous expression system of the human ferritin H-chain gene (hfH) allowed us to characterize the cellular effects of ferritin in yeasts. The recombinant Saccharomyces cerevisiae (YGH2) evidenced impaired growth as compared to the control, which was correlated with ferritin expression and with the formation of core minerals. Growth was recovered via the administration of iron supplements. The modification of cellular iron metabolism, which involved the increased expression of high-affinity iron transport genes (FET3 and FTR1), was detected via Northern blot analysis. The findings may provide some evidence of cytosolic iron deficiency, as the genes were expressed transcriptionally under iron-deficient conditions. According to our results examining reactive oxygen species (ROS) generation via the fluorescence method, the ROS levels in YGH2 were decreased compared to the control. It suggests that the expression of active H-ferritins reduced the content of free iron in yeast. Therefore, present results may provide new insights into the regulatory network and pathways inherent to iron depletion conditions.
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KEYWORD
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Expression, High-affinity iron transporter, Hferritin, Yeast
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